RT LAMP TEST

Subject: Science & tech

Concept:

About RT-LAMP Technology:

• RT- LAMP stands for Reverse Transcription Loop-Mediated Isothermal Amplification which produces a high DNA yield (>10μg).
• This method can produce results in significantly less time, which will increase the throughput of labs in India.
• The test uses an isothermal setup to create copies of viral DNA for detection, which significantly reduces the complexity of the overall process compared to the prevalent Real-Time PCR technology.
• This test also uses proprietary magnetic nanoparticle-based RNA extraction, which gives a highly purified and concentrated level of RNA.

Advantages:

• The LAMP technology has many advantages over RT-PCR technology. The RT-PCR test needs different temperatures in one cycle. The temperature of the solution has to be changed from 92 degrees C to 56 degrees C and again to 72 degrees C every two minutes, and this cycle has to be repeated. Thus, the PCR test needs expensive thermal cycler as well as the real time PCR machines.
• On the other hand, the new RT-LAMP technology is done at 65 degrees C, where the DNA amplification is done at a constant temperature (isothermal), so that expensive thermal cycler is not required.
• Moreover, the quantity of DNA amplified in the LAMP technology is hundred thousand times more than that is taking place in PCR.
• An equally important advantage of the LAMP technology is that the assay is so fast that results can be obtained within 30 minutes and positive samples are amplified as early as 10 minutes. .
• Yet another advantage of LAMP is that the reagents are to be stored at 4 degree C (ordinary fridge), whereas the PCR-based reagents are to be stored and transported at –20 degrees C, which needs deep freezers that escalate the cost.
• The LAMP technology does not need laborious preparation as in the case of RT-PCR. LAMP is cost effective and does not need complex expensive equipment. Assays can be performed with minimum skill and minimum infrastructure.